Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negative regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0018	PA4462 (RpoN)	T6SS00003 ; T6SS00004 ; T6SS00005	protein	Global regulator	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	4992870..4994363 (+)	15599658	PA1512 (hcpA) promotor (↓); PA5267 (hcpB) promotor (↓)
REG0069	PA4462 (rpoN)	T6SS00004	protein	Global regulator	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	4992870..4994363 (+)	15599658	T6SS transcript (↑)
REG0075	PP_0952 (RopN)	T6SS00014	protein	c-di-GMP regulatory pathway	Pseudomonas putida KT2440	chromosome (NC_002947)	1094567..1096060 (-)	26987688	PP_4373 (FleQ) (↑)
REG0095	ECA0288 (rpoN)	T6SS00040	protein	Global regulator	Pectobacterium atrosepticum SCRI1043	chromosome (NC_004547)	336007..337440 (-)	499404454	T6SS gene cluster promoter (PECA3445) (↓); Hcp gene cluster promoter (PECA4275) (↓); Hcp gene cluster promoter (PECA2866) (↓)
REG0124	VFFQA001_02105 (rpoN)	T6SS01277	protein	Global regulator	Aliivibrio fischeri strain FQ-A001	chromosome (NZ_SJSX01000002)	62995..64464 (-)	501526930	hcp1/hcp transcript (↓)
REG0182	VC0395_A2109 (rpoN)	T6SS00208	protein	Global regulator	Vibrio cholerae O395	chromosome (NC_009457)	2265078..2266541 (+)	446727517	T6SS gene cluster promoter (PVCA0107/VC0395_RS00160) (↓); hcp promoter (PVCA0017/VC0395_0116) (↓)
REG0184	Mmwyl1_2418 (RpoN)	T6SS00216	protein	Global regulator	Marinomonas sp. MWYL1	chromosome (NC_009654)	2725212..2726687 (+)	501017436	T6SS gene cluster promoter (PMWYL1196/PMWYL1195) (↓)
REG0300	VCA1552_RS11610 (RpoN)	T6SS01112	protein	Global regulator	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	2488023..2489486 (+)	446727517	Hcp expression and secretion (↓)
REG0317	VCV52_RS13040 (rpoN)	T6SS00790	protein	Global regulator	Vibrio cholerae V52	chromosome (NZ_KQ410502)	216932..218395 (-)	446727517	Unknown (↓)
REG0345	BN49_RS03755 (rpoN)	T6SS01214	protein	Global regulator	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	533564..534997 (-)	489007850	TssB transcription (↓)
REG0354	AHA_3923 (rpoN)	T6SS00157	protein	Global regulator	Aeromonas hydrophila subsp. hydrophila ATCC 7966	chromosome (NC_008570)	4350676..4352115 (-)	117618923	T6SS gene cluster promoter (PAHA1826) (↓)
REG0074	PP_4373 (FleQ)	T6SS00014	protein	c-di-GMP regulatory pathway	Pseudomonas putida KT2440	chromosome (NC_002947)	4963882..4965357 (-)	26991062	K1-T6SS (hcp1) expression (↑)